Skin science article
GHK-Cu Dosage Chart: 50 mg Vial Protocol
GHK-Cu (50 mg Vial) Dosage Protocol GHK-Cu Dosage Chart This GHK-Cu dosage chart explains a 50 mg vial prepared to a final volume of 3.0 mL for a concentration of 16.67 mg/mL. The GHK-Cu dosage protocol examples below cover 1.0 mg, 1.5 mg, and 2.0 mg amounts,
GHK-Cu (50 mg Vial) Dosage Protocol
GHK-Cu Dosage Chart
This GHK-Cu dosage chart explains a 50 mg vial prepared to a final volume of 3.0 mL for a concentration of 16.67 mg/mL. The GHK-Cu dosage protocol examples below cover 1.0 mg, 1.5 mg, and 2.0 mg amounts, with matching U-100 syringe units, reconstitution steps, supplies, and research context.
Reconstitute: Prepare the 50 mg vial to a final volume of 3.0 mL for a concentration of 16.67 mg/mL.
Research protocol range: The tables below cover 1.0 mg, 1.5 mg, and 2.0 mg using the standard and alternative research-use schedules.
Easy measuring: At 16.67 mg/mL, 1 U-100 unit = 0.01 mL = approximately 166.7 mcg.
Quick conversions: 1.0 mg = 6 units, 1.5 mg = 9 units, and 2.0 mg = 12 units.
GHK-Cu, or glycyl-L-histidyl-L-lysine copper complex, is a naturally occurring copper peptide studied for collagen-related pathways, tissue remodeling, antioxidant defense, and skin repair[7][8]. This educational GHK-Cu dosage protocol presents the same research-use schedule for the 50 mg vial, with calculations adjusted for its concentration.
Research context: For evidence on mechanisms, human and preclinical research, limitations, and safety, read GHK-Cu Peptide: Benefits, Uses, Side Effects, Dosage, and Research.
Standard / Conservative Approach (3 mL = 16.67 mg/mL; 5 days/week)
Weeks 1-4
1.0 mg (1000 mcg)
6 units (0.06 mL)
Weeks 5-8
1.5 mg (1500 mcg)
9 units (0.09 mL)
Weeks 9-12+
2.0 mg (2000 mcg)
12 units (0.12 mL)
Frequency: Once daily, 5 days per week, progressing from 1.0 mg to 1.5 mg and then 2.0 mg as shown above. At 16.67 mg/mL, those doses equal 6, 9, and 12 U-100 units[18]. Use a U-100 syringe with markings that clearly display every value in the table.
Reconstitution Steps
Draw 3.0 mL of the protocol-specified sterile diluent with a sterile syringe.
Add it slowly down the vial wall to reduce foaming.
Gently swirl or roll until dissolved. Do not shake.
Label the vial with the 16.67 mg/mL concentration, preparation date, and storage instructions.
Alternative Protocol (3 mL = 16.67 mg/mL; 3 times weekly)
Weeks 1-12+
Frequency: 2.0 mg three times per week, such as Monday, Wednesday, and Friday. At 16.67 mg/mL, each injection equals 12 U-100 units (0.12 mL), for approximately 6 mg per week[18].
Supplies Needed
Plan based on an 8-16 week protocol. Calculations are shown for both the 5 days/week and 3 times/week schedules.
Peptide Vials (GHK-Cu, 50 mg each):
5 days/week (1.0-2.0 mg/day):
8 weeks (~50 mg total): 1 vial
12 weeks (~90 mg total): 2 vials
16 weeks (~130 mg total): 3 vials
3 times/week (2 mg each):
8 weeks (~48 mg total): 1 vial
12 weeks (~72 mg total): 2 vials
16 weeks (~96 mg total): 2 vials
Insulin Syringes (U-100, 29-31 gauge):
5 days/week:
Per week: 5 syringes
8 weeks: 40 syringes
12 weeks: 60 syringes
16 weeks: 80 syringes
3 times/week:
Per week: 3 syringes
8 weeks: 24 syringes
12 weeks: 36 syringes
16 weeks: 48 syringes
Sterile or Bacteriostatic Water (10 mL bottles): Use 3.0 mL per vial for the displayed calculation.
1 vial: 3 mL
2 vials: 6 mL
3 vials: 9 mL
Alcohol Swabs: Two administration swabs per injection: one for the vial stopper and one for the injection site.
Per week: 10 swabs
8 weeks: 80 swabs or 1 x 100-count box
12 weeks: 120 swabs or 2 x 100-count boxes
16 weeks: 160 swabs or 2 x 100-count boxes
Per week: 6 swabs
8 weeks: 48 swabs or 1 x 100-count box
12 weeks: 72 swabs or 1 x 100-count box
16 weeks: 96 swabs or 1 x 100-count box