Ingredient or product comparison
Comparison: GHK-Cu Purity Verification Methods
HPLC Chromatography Total purity. Separates peptide from synthesis byproducts and truncated sequences ≥98% purity Cannot confirm molecular identity. Only that something elutes at the expected time Required. This is the gold standard for quantifying peptide pur
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- HPLC Chromatography
- Total purity. Separates peptide from synthesis byproducts and truncated sequences
- ≥98% purity
- Cannot confirm molecular identity. Only that something elutes at the expected time
- Required. This is the gold standard for quantifying peptide purity
- Mass Spectrometry (MS)
- Molecular weight confirmation to four decimal places
- Exact mass match within ±0.05 g/mol
- Cannot quantify how much of the sample is GHK-Cu versus contaminants
- Required. Confirms identity but not concentration
- UV Spectroscopy
- Presence of aromatic amino acids or copper coordination
- Qualitative only. Presence/absence
- Cannot differentiate GHK-Cu from structurally similar peptides
- Insufficient alone. Useful as a preliminary screen but not proof of purity
- LAL Endotoxin Testing
- Bacterial contamination (lipopolysaccharides)
- <0.5 EU/mg
- Does not detect chemical impurities or peptide degradation products
- Required for research use. Endotoxins compromise reproducibility in biological assays
- ICP-MS Copper Content
- Copper ion stoichiometry. Verifies copper is bound to peptide
- 1:1 molar ratio (one copper per tripeptide)
- Cannot confirm the copper is coordinated to the peptide versus present as free salt
- Critical for GHK-Cu specifically. Free copper negates the peptide's documented effects