Ingredient or product comparison
Comparison: GHK-Cu Verification Methods
HPLC-MS Molecular weight, retention time, purity by area Confirms intact GHK-Cu at 340 Da, distinguishes from degraded fragments and deletion sequences Does not verify copper coordination or peptide:copper ratio Gold standard. Definitively confirms peptide ide
This source-based comparison does not add ratings or recommend a winner.
- HPLC-MS
- Molecular weight, retention time, purity by area
- Confirms intact GHK-Cu at 340 Da, distinguishes from degraded fragments and deletion sequences
- Does not verify copper coordination or peptide:copper ratio
- Gold standard. Definitively confirms peptide identity and purity
- $150–300 per sample
- Amino Acid Analysis
- Individual amino acid concentrations after hydrolysis
- Verifies Gly:His:Lys ratio is 1:1:1, confirms peptide bonds are intact
- Cannot detect copper dissociation or distinguish stereoisomers
- Essential for confirming sequence integrity
- $100–200 per sample
- ICP-MS Copper Quantification
- Total copper content in the sample
- Confirms peptide:copper stoichiometry is 1:1, detects free copper contamination
- Does not verify peptide structure or sequence
- Critical for verifying functional copper coordination
- $80–150 per sample
- UV-Vis Spectrophotometry
- Absorbance at 280 nm (peptide bonds) and 620 nm (copper)
- Estimates total peptide and copper concentration
- Cannot distinguish GHK-Cu from degraded peptides or deletion sequences. All absorb similarly
- Inadequate for purity verification. Useful only for rough concentration estimates
- $20–50 per sample
- Visual Inspection + pH Testing
- Color, clarity, pH range
- Confirms product is blue-tinted and within pH 6.5–7.5
- Tells you nothing about peptide integrity. Degraded products look identical
- Useless for purity. Appearance proves nothing
- Free