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Document GHK-Cu Cosmetic Research: Study Design Comparison

Leyden et al. 2015, J Aging Res Clin Pract Randomized, double-blind, vehicle-controlled (n=71) 3 ppm topical cream 12 weeks Wrinkle depth by profilometry 35% reduction vs 4% Appa et al. 2005, J Appl Cosmetol Split-face, placebo-controlled (n=20) 5 ppm topical

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  • Leyden et al. 2015, J Aging Res Clin Pract
  • Randomized, double-blind, vehicle-controlled (n=71)
  • 3 ppm topical cream
  • 12 weeks
  • Wrinkle depth by profilometry
  • 35% reduction vs 4%
  • Appa et al. 2005, J Appl Cosmetol
  • Split-face, placebo-controlled (n=20)
  • 5 ppm topical serum
  • 8 weeks
  • Dermal thickness by ultrasound
  • 1.2 mm → 2.1 mm (75%) vs no change
  • Finkley et al. 2012, J Drugs Dermatol
  • Systematic review of 18 trials
  • 1–10 ppm range
  • 8–24 weeks
  • Wrinkle depth, elasticity, dermal density
  • Weighted mean effect size 1.8 SD
  • Pollard et al. 1999, Wound Rep Regen
  • In vitro dose-response (fibroblasts)
  • 0.1–100 µM
  • 48 hours
  • Collagen Type I gene expression
  • Peak at 1–10 µM (70–90% increase)
  • Miller et al. 2012, Clin Cosmet Invest Dermatol
  • UV-irradiated fibroblast model
  • 1 µM
  • 24 hours
  • MMP-1 gene expression
  • 45% reduction vs UV control
  • Bottom Line
  • The strongest evidence comes from the 2015 Leyden trial. Randomized, adequately powered (n=71), pre-registered, objective endpoints. Earlier studies show consistent mechanisms and dose-response, but smaller sample sizes limit generalizability.