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Ingredient or product comparison

GHK-Cu and TB-500 Stack: Research Protocol Comparison

Biomedicine & Pharmacotherapy 2020 Diabetic mouse excisional wounds 500 µg topical 200 µg topical Once daily × 14 days Wound closure % at day 14 89% closure (combination) vs 67% (GHK-Cu alone) vs 71% (TB-500 alone) vs 48% (saline) Int J Mol Sci 2021 Human fibr

This source-based comparison does not add ratings or recommend a winner.

  • Biomedicine & Pharmacotherapy 2020
  • Diabetic mouse excisional wounds
  • 500 µg topical
  • 200 µg topical
  • Once daily × 14 days
  • Wound closure % at day 14
  • 89% closure (combination) vs 67% (GHK-Cu alone) vs 71% (TB-500 alone) vs 48% (saline)
  • Int J Mol Sci 2021
  • Human fibroblast collagen gel contraction
  • 10 µM in media
  • 50 nM in media
  • Continuous exposure × 7 days
  • Gel contraction %
  • 64% contraction (combination) vs 32% (GHK-Cu) vs 19% (TB-500) vs baseline
  • J Invest Dermatol 2019
  • Keratinocyte scratch assay
  • 5 µM in media
  • 100 nM in media
  • Continuous exposure × 48 hours
  • Migration velocity (µm/hr)
  • 31 µm/hr (combination) vs 18 µm/hr (TB-500) vs 12 µm/hr (GHK-Cu) vs 12 µm/hr (control)
  • Professional Assessment
  • All three studies used mechanistically distinct endpoints (closure, contraction, migration) yet showed consistent synergistic effects in the 40–60% improvement range when combining GHK-Cu and TB-500 compared to individual peptides. The dosing across models converged on GHK-Cu at 1–10 µM and TB-500 at 10–100 nM. Concentrations that align with receptor binding affinities for their respective targets.